Analysis of Protein-Protein Interface Analysis of Protein-Protein Interface

 


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Specify chain ids in components 1 and 2:

[ Default: A for Component 1 and B for Component 2 ]

Component 1 Component 2
Chain 1 Chain 2 Chain 3
Chain 1 Chain 2 Chain 3

Threshold for clustering: [ recommended value
15.0 A for complexes, 22.0 A for homodimers ]

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Citations

[1] Saha R. P., Bahadur R. P., Pal A., Mandal S. and Chakrabarti P. (2006). ProFace: A server for the analysis of the physicochemical features of protein-protein interfaces. BMC Struct. Biol. 6: 11.

[2] Bahadur R. P., Chakrabarti P., Rodier F. and Janin J. (2004). A dissection of specific and non-specific protein-protein interfaces. J. Mol. Biol. 336, 943-955.

[3] Hubbard S. J. (1992). NACCESS: A program for calculating accessibilities. Department of Biochemistry and Molecular Biology. University College of London. http://wolf.bms.umist.ac.uk/naccess/.

[4] Kabsch W. & Sander C. (1983). Dictionary of protein secondary structure. Pattern recognition of hydrogen-bonded and geometrical features. Biopolymers 22, 2577-2637. http://www.cmbi.kun.nl/gv/dssp.

Instructions

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Once the JRE has been properly set up on your computer, you will be prompted to accept security certificates signed by "Dr. Pinak Chakrabarti" when your browser attempts to launch the applets. This is to enable uploading of files by the users. No personal information will be retained from the user's computer on our server other than the data he/she voluntarily submits, which will be deleted periodically.

The users should submit the PDB files in plain text format only. Any other file format will not be accepted by our server and you will receive an error message. The filename can be of any length, but the extension (following the period) has to be of exactly three characters. There is also an upper limit of 2 MB on the filesize. Once the file is submitted, several programs are run on it on our server and the user ultimately receives a single web page showing the secondary structure information for all the chains as provided by the DSSP program. Additionally, all the interface residues are shown and the user has the option of dissecting the residues into patches or core/rim regions. The software expects the residue numbers (against the co-ordinate records) in the PDB file to be in serial order. Negative residue numbers or using using the same residue number for multiple residues will cause problems.

Under heavy traffic conditions and depending on the size of your PDB file, you might be required to wait as long as 10 minutes from the submission of the PDB file to the display of the results. If you have any query, please drop a mail to to Dr. Pinak Chakrabarti (pinak@bic.boseinst.ernet.in).

Disclaimer

The software is provided as is. Though the software has been tested successfully on a large number of files, we cannot guarantee that the system is fool-proof. Any comments or suggestions for improving the software or this site are welcome.

External Software Used

Public Domain:

NACCESS

Correspondence to:

Dr. Simon Hubbard
Biochemistry & Applied Molecular Biology
UMIST, PO Box 88,
Manchester M60 1QD, UK
E-mail: sjh@sjh.bi.umist.ac.uk

DSSP

Correspondence to:

Centre for Molecular and Biomolecular Informatics
University of Nijmegen, Toernooiveld 1, P.O. Box 9010,
6500 GL Nijmegen, Netherlands
E-mail: postmaster@cmbi.kun.nl

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Page Last Updated On : 16/7/2007 by Webmaster